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Salmonella Detection methodologies vary depending on the bacterial species in question.
These two species are species specific and are pathogenic microorganisms for birds. Both can be devastating to the poultry industry, so detection of these microorganisms is critical. The focus with both types of bacteria should always to detect them in the birds themselves, either the breeders or the progeny. Both SG and SP can cause systemic infections, so culture specimens should include:
It is common for the organs and tissues that represent each of these systems to be pooled for culture. Before removing the tissues to prepare separate organ pools, blood agar or other non-selective media should be inoculated directly with MacConkey agar (MAC) from any organ showing macroscopic lesions.
SG and SP are not as tolerant to high temperatures as other species of salmonella. Therefore, they should not be cultivated at 42 ° C during the first 24 hours before being inoculated in plates of slightly selective culture medium. In contrast, in mobile Salmonella cultures, incubation is carried out at 42 ° C in these first 24 hours.
At the same time, direct sowing is done on selective media, such as bright green supplemented with novobiocine, commonly known as BGN. If you are also looking for other salmonella species such as S. enteritidis (SE), then cultures on XLT4 agar plates (Xylose, Lysine, Tergitol) should be added.
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